Equipment

Cytek Aurora

Description: Cell Analyzer

67 Channel System + Plate Loader

5 Laser UV/V/B/YG/R

64 Fluorescence Channels, 3 Scattering Channels (FSC, blue laser SSC, violet laser SSC)

Spectraflo Software

Optics

Lasers: Base model three-laser configuration: 405 nm: 100 mW, 488 nm: 50 mW, 640 nm: 80 mW

Beam Geometry: Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.

Emission Optics

Emission Collection: Fused silica cuvette coupled to high NA lens for optimum collection efficiency to optical fibers.

Forward and Side Scatter Detection:  

FSC: high-performance semiconductor detector with 488nm bandpass filter 

SSC: two high-performance semiconductor detectors with 405nm and 488nm bandpass filters

Fluorescence Detectors: Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser enabling more efficient spectrum capture in the 365-829 nm range. No filter changes required for any fluorochrome excited by the 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers

Standard Optical Configuration:

Violet detector module: 16 channels unevenly spaced bandwidth from 420-829 nm. 

Blue detector module: 14 channels unevenly spaced bandwidth from 498-829 nm. 

Red detector module: 8 channels unevenly spaced bandwidth from 652-829 nm. 

4 and 5 Laser Options: Yellow-Green detector module: 10 channels unevenly spaced bandwidth from 567-829 nm. 

Ultraviolet detector module: 16 channels unevenly spaced bandwidth from 365-829 nm.

Fluidics

Sample Flow Rates: 

Low: 15 µL/min

Medium: 30 µL/min

 High: 60 µL/min, Plate high-throughput mode: 100 µL/min

Fluidic Modes: Long clean, SIT flush, Purge filter, Clean flow cell

Manual Sample Input Formats: 12x75 mm polystyrene and polypropylene tubes

Standard Fluidic Reservoirs: 4L fluid container set with level-sensing provided. Compatible with 20 L sheath and waste cubitainers.

Volumetric Sensor: Volumetric measurement during sample recording enables calculation of counts per µL for any gated population.  

Plate Loader Options

Plate stage temperature: 4-30°C (AMS only)

High Throughput Speed: ASL Loader: 27 min for 96-well plate AMS Loader: 35 min for 96-well plate

Input Compatibility: 

ASL Loader: 27 min for 96-well plate 

AMS Loader: 35 min for 96-well plate

Plate Loader Carryover

Default mode: ≤0.3%, Low Carryover mode: ≤0.1%, High Throughput mode: ≤1%

Performance

Fluorescence Linearity: FITC R2 ≥0.995 / PE R2 ≥0.995

Forward and Side Scatter Resolution: Performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Carryover: ≤0.1%

Data Acquisition Rate: 35,000 events/s (Three laser system)

Software

Live unmixing during acquisition 

Developed specifically to streamline assay setup, data acquisition, and file export 

Automated QC module 

Autofluorescence extraction 

Raw and Unmixed FCS 3.1 files



 

Licor Odyssey CLx Odyssey

Description: Western Blot Imager

Image Field Size: 25 cm W × 25 cm D (9.8” W × 9.8” D)

Resolution: 21-337 µm

Dynamic Range*: Manual: >4 logs, Auto: >6 logs

Detectors: Silicon avalanche photodiodes

Laser Lifetime: 40,000 hours of operation, Class 1 Laser Product

Light Sources:

Solid-state diode laser at 685 nm
Solid-state diode laser at 785 nm

* This dynamic range is obtained in a single acquisition.

  Specifications subject to change without notice.

 

Thermo Fisher Nanodrop 3300 (OneC Microvolume)

Description: Nanodrop Spectrophotometer

Full spectrum microvolume UV-VIS Measurements with a cuvette option in a single instrument. 

Sampling Options: Pedestal and cuvette

Measures: 1-2ul sample

Dynamic range: (ng/ul dsDNA) 2.0 - 27,500* ng/ul for dsDNA

Full spectra data

Pre-programmed methods for nucleic acids and proteins

Measures nucleic acid: A260

Measures nucleic acid: A260/A230 ratio

Measures purified protein: A280

Measures protein and peptides at: A205

Measures protein: A260/A280 ratio

Preprogrammed methods for colorimetric assays (BCA, Bradford, Lowry. Pierce 660)

Custom methods

Built-in cuvette option

Standalone local control

High-resolution touchscreen interface

Measures fluorescent-labeled nucleic acid and protein samples using absorbance

Autoblank and Automeasure

Acclaro contaminant identification

 

BioRad CFX Opus 384

Description: RT-PCR (384)

Thermocycler

Maximum Ramp Rate, °C/sec: 2.5

Average Ramp Rate, °C/sec: 2

Heating And Cooling Method: Peltier

Lid, °C: 30–110

Temperature

Range, °C: 4–100

Accuracy, °C: ±0.2 of programmed target at 90°C

Uniformity, °C: ±0.3 well-to-well after 10 sec of arrival at target

Gradient

Operational range, °C: 30–100

Programmable span, °C: 1–24

Optical Detection

Excitation: 5 filtered LEDs

Detection: 5 filtered photodiodes

Range of Excitation Wavelengths, nm: 450–650

Range of Emission Wavelengths, nm: 515–690

Sensitivity: Detects 1 copy of target sequence in human genomic DNA

Dynamic Range: 10 orders of magnitude

Scan Time

All Channels, sec: <20

FAM/SYBR® Green Only, sec: 8

System

Sample Capacity, Wells: 384

Sample Size, µl: 1–30 (5–30 recommended)

Communication Interface: USB 2.0

 

BioRad CFX Opus 96

Description: RT-PCR (96)

Thermocycler

Maximum Ramp Rate, °C/sec: 5

Average Ramp Rate, °C/sec: 3.3

Heating And Cooling Method: Peltier

Lid, °C: 30–110

Temperature

Range, °C: 4–100

Accuracy, °C: ±0.2 of programmed target at 90°C

Uniformity, °C: ±0.3 well-to-well after 10 sec of arrival at target

Gradient

Operational range, °C: 30–100

Programmable span, °C: 1–24

Optical Detection

Excitation: 6 filtered LEDs

Detection: 6 filtered photodiodes

Range of Excitation Wavelengths, nm: 450–684

Range of Emission Wavelengths, nm: 510–730

Sensitivity: Detects 1 copy of target sequence in human genomic DNA

Dynamic Range: 10 orders of magnitude

Scan Time

All Channels, sec: 12

FAM/SYBR® Green Only, sec: 3

Software

Operating Sysetem: Windows 10

Multiplex Analysis: Up to 5 targets per well

System

Sample Capacity, Wells: 96

Sample Size, µl: 1–50 (10–50 recommended)

Communication Interface: USB 2.0

 

Fisher Scientific HM525 NX

Description: High Performance Routine Cryostat

Ergonomic/Safety features

Mechanical handwheel lock

Optional UV disinfection

Automatic specimen retraction on return stroke

Touch screen operation with intuitive software to accommodate multiple users

Cryochamber features

Chamber cooling to -35°C

27 cryobar storage positions including 4 quick freeze locations

Integrated peltier fast freezing device rapidly cools to -57°C ± 3°C

Programmable and immediate defrost options

Microtome features

Manual light touch rotary flywheel

Section thickness from 1µm to 500µm

Memory function for rapid advance and specimen positioning

Vertical stroke length of 60mm28mm horizontal feed range

40μm specimen retraction on return stroke

XY specimen orientation with 360° z axis rotation

Chamber Temperature: -35°C +/-3°C Max. at +20°C Room Temperature

Defrost: Programmed/ Immediate

Heidolph HEI-VAP Expert Control ML/G3B

Description: Rotary Evaporator

Rotation Speed Indicator: 5" LCD color graphic display 

Permissible Ambient Conditions: 5 – 31 °C at 80 % rel. humidity 32 – 40 °C decreasing lineary up to max 50% rel. humidity 

Protection Class: IEC 60529 IP 20

Lift: Hand lift or Motor lift 

Height Adjustment Speed: Hand lift models: manual Motor lift models: 25 mm/s 

Height Adjustment: 155 mm 

Drive: brushless DC motor with electronic speed control Heating capacity 1,300 W

Temp. Range Heating Bath: 20 – 210 °C 

Temperature Accuracy Bath: ±1 °C 

Overheat Protection Bath: cut-off at 5 °C over set temperature via separate PT 1000 

Bath Temperature Setting: 5" LCD color graphic display Heating bath temperature control electronic / digital 

Secondary Overtemp. Cut Off: 250 °C 

Material Heating Bath: stainless steel V4A 

Diameter Heating Bath: 255 mm 

Volume Heating Bath: 4.5 l 

Display Vapor Temperature: Yes 

Required Accessories For Display Vapor Temperature: Vapor temperature sensor 

Integrated vacuum controller:

Timer: Yes 

Programmable Ramps: Yes 

Supply Power: 1,400 W

Max. Size Evaporating Flask: 5 L 

Rotation Speed Range: 10 - 280 rpm 

Solvent Libary: Yes

Perkin Elmer In Vivo Imaging System (IVIS) Inst. Spectrum 120V Andor Camera

Description: IVIS Spectrum

High-sensitivity in vivo imaging of fluorescence and bioluminescence

High throughput (5 mice) with 23 cm field of view

High resolution (to 20 microns) with 3.9 cm field of view

Twenty eight high efficiency filters spanning 430 – 850 nm

Supports spectral unmixing applications

Ideal for distinguishing multiple bioluminescent and fluorescent reporters

Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination

3D diffuse tomographic reconstruction for both fluorescence and bioluminescence

Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.

NIST traceable absolute calibrations

Gas anesthesia inlet and outlet ports

Class I Laser Product

 

Agilent 8453 UV-Visible Spectrophotometer

Description: UV-VIS

Optical Specifications

Wavelength Range: 190–1100 nm 

Slit Width: 1 nm

EP Resolution Test: >1.6    spectrum of a 0.02% v/v solution of toluene in hexane, ratio absorbance at 269 nm/266 nm 

Stray Light: 

<0.03% at 340 nm (NaNO2, ASTM)

<0.05% at 220 nm (NaI, ASTM) 

<1% at 200 nm (KCl, EP)

Wavelength Accuracy: <±0.5 nm (NIST 2034)* <±0.2 nm at 486.0 and 656.1 nm 

Wavelength Reproducibility: <±0.02 nm ten consecutive scans (NIST 2034)

Photometric Accuracy: 

<±0.005 A at 1 A (NIST 930e) <±0.01A potassium dichromate, EP method 

Photometric Noise: <0.0002 A sixty 0.5-s scans at 0 A, 500 nm, rms 

Photometric Stability: <0.001 A/h at 0 A, 340 nm, after 1 h warmup, measured over 1 h, every 5 s, constant ambient temperature

Baseline Flatness: <0.001 A 0.5-s blank, 0.5-s scan, rms Typical scan time 1.5 s full range 

Environmental Conditions

Operating Temperature: 0–50 °C (32–122 °F) 

Non-Operating Temperature: -40–70 °C (-4-–58 °F) 

Humidity: <95 %, at 25–40 °C (77–104 °F) 

 

Labconco Freezone Triad

Description: Benchtop Freeze Dryer (Lyophilizer)

Stainless steel collector coil capable of removing 1.84 L of water in 24 hours and holding 2.5 L of ice before defrosting* 

Two 1/3 hp HCFC/CFC-free refrigeration compressors cool the collector to -85° C (-121° F) and work in concert with a 1000-watt heater to cool and heat fluid medium circulating through channels in the shelf. 

Not for use with samples containing methanol or ethanol 

Processing shelf, 12.4" w x 14.5" d (31.5 x 36.8 cm). Spacing between the shelf and top of the chamber accommodates containers with stoppers up to 148 mm maximum height 

One probe for monitoring the temperature of the sample in the chamber 

Pirani vacuum sensor 

Temperature ramp and hold programming from -55° C to +50° C (-67° F to +122° F) and -75° C (-103° F) during pre-freezing. Controls temperature to ±1° C of set point. Memory to store 30 programs with 16 segments each including a number of steps, ramp rate, shelf temperature, hold time, and vacuum level 

Full color, 5" capacitive touch screen with Lyo-Works OS: – Real time display of collector temperature and vacuum level – Creation and storage for up to 30 programs with up to 16 steps each. Ability to set ramp rate, shelf temperature, shelf temperature time, and vacuum set point per step – Manual start-up buttons for collector and shelf temperature control and vacuum pull down – Alerts, diagnostic tests, user manual and defrost control – Data logging stores and displays data in table or graph format, and may be exported via USB or Ethernet – Settings include language (English, Spanish, French, German, or Italian), time, temperature (° C or ° F) and vacuum (mBar, Torr or Pa) – Alerts can be emailed to user-entered addresses via Ethernet connection – Vacuum pump maintenance alert – Security lock-out 

Pneumatic stoppering mechanism with diaphragm and plate. No compressed gas is required 

Stoppering control knob, vacuum release control knob, and 1/8" OD user-supplied sterile/inert gas backfill port 

Four left side-mounted neoprene valves with molded plastic knobs and fittings that accommodate 1/2" and 3/4" flask adapters

Electrical receptacle for vacuum pump 

Hot gas defrost with the stainless steel drain pan, silicone drain hose and auto shut off at +60° C (+140° F) 

Vacuum control valve 

Vacuum break valve 

Stainless steel and powder-coated steel exterior 

Clear acrylic door, 1" thick, with silicon gasket and latch 

3/4" OD vacuum connection. 36" long, 3/4" ID vacuum hose with two clamps

Serum Bottle Capacity 

Catalog Number

7575010

7575210

7575310

7573210

7573410

7573610

7573610 

7574010 

7574210

Volume

2 ml

3 ml

5 ml

10 ml

20 ml

30 ml

50 ml

100 ml

125 ml

Shelf Capacity

400

441

233

196

121

86

64

42

36

FreeZone® Triad® Benchtop Freeze Dryers Specifications *Freeze drying rate will be lower for samples other than plain water. For optimum performance, room temperature should be 21° C (70° F) or colder.




 

Evos M5000

Description: Imaging System/ Microscope

Camera: 3.2 Megapixels, Monochrome, CMOS

For Use With (Applications): Imaging

For Use With (Equipment): EVOS™

Light Source: LED

Magnification: 1.25X to 100X

Objectives: 5 Position Turret

Resolution: 1920 x 1080 pixels

Display: 18.5 in Articulated LCD

Dimensions: 18 in (W) x 23 in (H) x 18 in (D)

 

Agilent Biotech Synergy Neo2 Hybrid Multi-Mode SMB

Description: Plate Reader

Automation Compatibility: BioStack, BioSpa, BenchCel, 3rd-party

Environment Control: Condensation Control, CO2, O2 Control, 4-Zone Incubation to 70 ºC 

Filter Detection Modes: Fluorescence Intensity, Fluorescence Polarization, BRET, Luminescence, TR-FRET, Time Resolved, Fluorescence (Laser), Alpha (Laser)

Imager/Reader Options: Barcode Reader, Reagent Injectors

Instrument Control:  Computer Software

Monochromator Detection Mode: UV-Vis Absorbance, Fluorescence Intentsity (variable bandwidth)

Shaking: Double-orbital, Linear, Orbital

Supported Labware:  6 to 1536-well plates, Take3/Take3 Trio, Petri/Cell culture dishes






 

Fisher Scientific Heracell 150i Dual SST TC

Description: Tissue Culture Dual Chamber Incubators 

Electrical Requirements: 120 V, 50/60 HZ

Chamber Material: Copper

CO2 Concentrations Range: 0 to 20%

CO2 Sensor Technology: TC Sensor

Lighting: LED

O2 Concentration Range: Optional 1 to 21% or 5 to 90%

Oxygen Control: Optional 1 to 21% or 5 to 90%

Relative Humidity: <90% at 37°C

Temperature Range (Metric): Ambient +3°C to 55°C

Type: CO2 Incubator

For Use With (Application): General Purpose

Humidity Source: Integrated water reservoir

 

Nuaire Class II/A2 

Description: Biosafety Cabinets

Class: II Type A2

Air Cleaniliness: ISO-Class-5

Airflow Monitor: Pressure

Cabinet Construction: All welded stainless steel 16/18 GA, Type 304 pressure tight design

Control System:  AeroMax

Downflow FPM (m/s): 60fpm (0.30m/s)

Inflow fpm (m/s): 105 fpm (0.53 m/s)

Protection: Product, Personnel, Environment

Work Access:  8, 10 inches (203, 254 mm) 



 

Fisher Scientific Isotemp General Purpose Upright

Description: Refrigerator / Freezer Combo

Volts: 115 ~ 

Hz: 60 

AMPS: 6.5 

Phase: 1

Refrigerant Type: R134A

 

VWR Freezer

Description: Standard -20C Freezer

Volts: 115  

Hz: 60 

AMPS: 5.0 

Phase: 1

Refrigerant Type: R134A

 

Refrigerated Centrifuge (5910 R)

Capacity: 4 x 1000 mL, 4 x 5 MTP

Max. Speed: 14,000 rpm

Refrigerated: Yes

Includes: Rotor S-4 x Universal, universal buckets and adapters for 5 mL/ 15 mL/ 50 mL connical tubes, 175 to 250 mL bottles and plates

Noise Level: <53 db(A)

Rotor Included: Yes

Temperature Range: -11°C to +40°C

Max. RCF: 22,132 x g

Safety Features: Lid lock, Steel armored safety chamber, View port for speed validation, Lid emergency release, Automatic rotor recognition, Certified aerosol-tight rotor lids and aerosol caps, Rotor lifecycle counter, Automatic imbalance recognition

Agilent High-Performance Liquid Chromatography – Analytical 1260 Infinity II_HPLC

Description: HPLC Analysis

m/z Range: n/a

Available detectors: UV-VIS

Sample Loop Size (ul): 100

Column: Poroshell 120 EC-C18, 3.0 x 100mm, 2.7um

Maximum Pressure (bar): 800

Size Of Fraction Collection Vials (ml): n/a

Flow Range (ml/min): 0.001-5

Column thermostat: yes 

Integrated degassing unit: yes

Automatic purge valve: yes

Gradient or isocratic: both

Mass accuracy: n/a

Software: Chemstation

Ion source: n/a

Practices: For targets that have chromophores

 

Agilent Liquid Chromatography Mass Spectrometry 1290 Infinity II_LC/MSD XT

Description: LC-MS 

m/z Range: 30-2950

Available Detectors: single quad, UV-VIS

Sample Loop Size (ul): 20

Column: Poroshell 120 EC-C18, 2.1x50mm, 1.9um

Maximum Pressure (bar): 1300

Size Of Fraction collection Vials (ml): N/A

Flow Range (ml/min): 0.001-5

Column Thermostat: yes 

Integrated Degassing Unit: yes

Automatic Purge Valve: yes

Gradient or Isocratic: both

Mass Accuracy: nominal

Software: Chemstation

Ion Source: ESI

Practices: For polar, acidic, & basic molecules

 

Agilent High-Performance Liquid Chromatography – Preparative 1260 Infinity II_FC-AS

Description: HPLC (Prep)

m/z Range: n/a

Available Detectors: UV-VIS

Sample Loop Size (ul): 100

Column: Poroshell 120 EC-C18, 4.6 x 100mm, 2.7um

Maximum Pressure (bar): 600

Size Of Fraction Collection Vials (ml): 2

Flow Range (ml/min): 0.001-5

Column Thermostat: yes 

Integrated Degassing Unit: yes

Automatic Purge Valve: no, manual

Gradient or Isocratic: both

Mass Accuracy: n/a

Software: Chemstation

Ion Source: n/a

Practices: For targets that have chromophores

 

Agilent Gel Permeation Chromatography 1260 Infinity II_GPC/SEC MDS

Description: GPC

m/z Range: 200-10M

Available Detectors: LS, RID, viscometer

Sample Loop Size (ul): 100

Column: none

Maximum Pressure (bar): 600

Size Of Fraction Collection Vials (ml): N/A

Flow Range (ml/min): 0.001-5

Column Thermostat: yes 

Integrated Degassing Unit: yes

Automatic Purge Valve: no, manual

Gradient or Isocratic: isocratic only

Mass Accuracy: average

Software: GPC/SEC

Ion Source: n/a

Practices: For macromolecules

Comment: for both organic & aqueous

Concern: extended time for solvent transfer and equilibration

 

Agilent Gas Chromatography-Mass Spectrometry 8890 GC/5977B MSD

Description: GC-MS

m/z Range: 1.6 - 1050

Available Detectors: single quad

Sample Loop Size (ul): n/a

Column: HP-5MS UI, 30m x 0.25mm ID x 0.25uM film

Maximum Pressure (bar): n/a

Size Of Fraction Collection Vials (ml): n/a

Flow Range (ml/min): n/a

Column Thermostat: yes 

Integrated Degassing Unit: n/a

Automatic Purge Valve: n/a

Gradient or Isocratic: both

Mass Accuracy: nominal

Software: MassHunter

Ion Source: EI

Practices: For volatile targets

Accessories: headspace sampler (HS)

Comments: Use is only for users with previous experience

Concerns: Worldwide helium shortage, no NIST database, HS sampler consumes much helium. 

 

EMD Millipore Milli-Q Reference

Description: Water Purification System

Conductivity: <100ppb

Contaminant Rejection (Microbiological): Less than 1cfu/ml

Feedwater Source: Elix Reverse Osmosis, Deionized, Distilled Water

Flow Rate: 50 and 2000 ml/min

Product Water Type: Type 1